Spr , PhD; Jean Dussault, MD; Paul J. Lupien, MD, PhD; Sital Moorjani, PhD; Andr?Nadeau, MD; Sylvie Pinault, MD; Jean-Aime Simoneau, PhD; and Germain Thriault, MD, and for their contributions to numerous elements of the research.Int J Obes (Lond). Author manuscript; obtainable in PMC 2014 August 01.Bouchard et al.Web page
Gaber et al. Chemistry Central Journal 2014, 8:33 http://journal.chemistrycentral/content/8/1/RESEARCH ARTICLEOpen AccessEnvironmentally evaluated HPLC-ELSD system to monitor enzymatic synthesis of a non-ionic surfactantYasser Gaber1, Cecilia Orellana erman2 and Rajni Hatti-Kaul2*AbstractBackground: N-Lauroyl-N-methylglucamide is actually a biodegradable surfactant derived from renewable resources. In an earlier study, we presented an enzymatic solvent-free method for synthesis of this compound. Inside the present report, the HPLC strategy created to adhere to the reaction involving lauric acid/methyl laurate and N-methyl glucamine (MEG) and its environmental assessment are described. Outcomes: Use of ultraviolet (UV) absorption or refractive index (RI) detectors didn’t allow the detection of N-methyl glucamine (MEG). With Evaporative light scattering detector ELSD, it was feasible to apply a gradient elution, and detect MEG with a limit of detection, LOD = 0.12 g. A superb separation in the peaks: MEG, lauric acid, product (amide) and by-product (amide-ester) was achieved with the gradient plan with a run time of 40 min. The setting of ELSD detector was optimized using methyl laurate because the analyte. LC-MS/MS was utilized to confirm the amide and amide-ester peaks. We evaluated the greenness of your developed system applying the freely readily available application HPLC-Environmental Assessment Tool (HPLC-EAT) and also the process got a scoring of 73 HPLC-EAT units, implying that the analytical process was more environmentally benign when compared with some other solutions reported in literature whose HPLC-EAT values scored as much as 182.131726-65-3 In stock Conclusion: Use of ELSD detector allowed the detection and quantification in the substrates as well as the reaction products of enzymatic synthesis with the surfactant, N-lauroyl-N-methylglucamide.5-Bromopyridine-2-carbaldehyde web The created HPLC approach has acceptable environmental profile based on HPLC-EAT evaluation.PMID:23829314 Keyword phrases: Green analytical chemistry, Chromatographic separation, Evaporative light scattering detector, Non-ionic surfactant, Mass spectrometry, HPLC-EATBackground N-Acyl-N-methyl glucamides, also known as fatty acid glucamides or alkyl glucamides (AGs), are non-ionic surfactants derived from glucose and fatty acids. They’re regarded as green chemical substances as a consequence of their renewable origin, biodegradability, and low environmental effect. The chemical structure of the AGs contains an amide bond among the hydrophobic along with the hydrophilic moieties (Scheme 1), which renders the molecule resistant for the alkaline circumstances, a desirable home in surfactants intended for detergent applications. Along with stability, security, compatibility and synergism with other surfactants, AGs were utilised in* Correspondence: [email protected] 2 Division of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden Complete list of author information is obtainable in the finish on the articlethe formulations of detergent, personal care, and pharmaceutical merchandise [1,2]. Industrial synthesis of fatty acid glucamides includes a two-step reaction: glucose reacts with methylamine in the initially step catalysed by Raney nickel.
